This room puts paintings and objects on display which testify to the spirituality and devotion of the Medieval Age.

Here the signs of the bishop's authority are shown through pieces dating to the XIII-XIV century. These express antique symbolic and formal characteristics taken from the European Romanesque tradition. The Miter and Pastoral staff are exceptional examples of the technical expertise in the use of yarns, enamels, and ivory. They can be seen as a symbol of the power of the Church in the world. An example of the artistic personality of the Master of Bellpuig is the panel depicting the Madonna del Latte (XIV century): the presence of this Catalan painter in the territory of Urbino and Montefeltro certaintly is an example of the abundance of artistic pursuit in European art, when the exchange of goods, men and ideas helped spread culture. Another work, Crocifisso (The Crucifix) by Antonio di Agostino da Fabriano (documented 1447-1489) follows in the tradition of large crosses painted for church apses, a symbol of universal piety.